Difference between revisions of "Microscopes"

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|location=Bay 3, 6 & 7
  
|location=Bay 3, 6 & 7
 
|description = Optical Microscopes
  
|description = Optical Microscopes
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|manufacturer = Nikon (3) & Olympus (1)
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|manufacturer = Nikon & Olympus
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}}[[UCSB NanoFab Microscope Training|'''Microscope Training''']] - Use this guide for general usage info & the many advanced features available on most of our microscopes.
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}}
 
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=Nikon=
  
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The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7. The scopes are connected to computers via Jenoptik ProfRes software which allows users to digitally capture images through the microscope.
  
   
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=Olympus=
 +
==Nikon==
  +
The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.
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The lab currently has one Olympus microscope (Microscope #1) which is located in bay 3.
  
   
  +
===Microscope #2: Nikon Optiphot 200 (Bay 7)===
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=Documentation=
  
  +
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*[[media:Nikon-Microscope-Manual.pdf|Nikon Microscope Instruction Manual (Ecplise)]]
  
  +
*Trinocular: Ocular Binoc. + Camera (Simultaneous)
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*[[media:Nikon-Microscope-OptiPhot-Manual.pdf|Nikon Microscope Instruction Manual (Optiphot)]]
  
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
−
*[[media:Jenoptik-ProgRes-Manual.pdf|Jenoptik ProgRes User Manual]]
  
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
**DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
  +
*Top Reflected Illumination (Episcopic)
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
  +
  +
===Microscope #3: Nikon Eclipse L200 (Bay 6)===
  +
  +
*[https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D]
  +
*Trinocular: Binoc. + Camera (Mutually Exclusive)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
*Top Reflected Illumination (Episcopic)
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
  +
  +
===Microscope #4: Nikon Eclipse L200D (Bay 6)===
  +
  +
*Trinocular: Binoc. + Camera (Mutually Exclusive)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
  +
  +
==Olympus==
  +
  +
The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.
  +
[[File:Microscope 01 Olympus BHMJL crop.png|alt=Photo of Microscope #01 in the Characterization lab, Room 1111|thumb|200x200px|Microscope #01 in the Characterization lab, Room 1111]]
  +
  +
===Microscope #1: Olympus BHMJL (Char. Lab, Room 1111)===
  +
  +
*Trinocular: Ocular Binoc. + Camera (Exclusive)
  +
*LED Illuminator, Variable
  +
*Objectives: ''10x, 20x, 50x, 100x''
  +
*Bright/Dark Field
  +
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for offline measurements: [https://www.amscope.com/software-download Link here].
  +
*User Manual: [https://www.nanotech.ucsb.edu/wiki/index.php/File:Olympus_-_BHM_Microscope_-_Instruction_Manual.pdf Olympus BHM Microscopes - Instruction Manual (PDF)]
  +
  +
===Microscope #5: [[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]]===
  +
  +
*See the wiki page for the [[Fluorescence Microscope (Olympus MX51)|Olympus MX51]] for full details
  +
*Trinocular: Binoc. + Camera (Simultaneous)
  +
*Native Olympus Stream Software:
  +
**Photo/video capture
  +
**Calibrated measurement (calibrations locked)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
*Top (Episcopic) & Bottom (Diascopic) Illumination
  +
*Three Fluorescence Filters (requires training, see main tool page for specs.)
  +
  +
===Microscope #6: [[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 7)]]===
  +
Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]]
  +
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  +
  +
*Motorized Stage + Objective Turret
  +
*Trinocular: Binoc. + Camera (Simultaneous)
  +
*Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  +
*Filters: ''to be added''
  +
*Native Olympus MX61 Software control & Camera
  +
**Calibrated measurements (calibrations locked)
  +
**Z (focus) measurement via motorized stage height
  +
*Deep-UV Light source + DUV Camera
  +
**DUV-100x sub-micron imaging/measurement
  +
  +
==[[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]]==
  +
See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]]
  +
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  +
  +
*Motorized stage + Objective Turret
  +
*100mm wafer stage
  +
*Native Olympus OLS2000 Software & Built-In Camera:
  +
**Calibrated measurement (calibrations locked)
  +
**Image stitching capabilities
  +
*3D Laser-Scanning Confocal Microscopy capability:
  +
**3D Topographical measurement (optical profilometry)
  +
**Surface roughness estimations (large roughness)
  +
**Thin-Film Film-Thickness Measurements (thicker films)
  +
  +
==Procedures & Documentation==
  +
  +
*[[UCSB NanoFab Microscope Training|'''Microscope Training''']] - General procedures and info for using our microscopes
  +
  +
*[//www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Nikon Microscope Instruction Manual (Ecplise)]
  +
*[//www.nanotech.ucsb.edu/wiki/images/2/28/Nikon-Microscope-OptiPhot-Manual.pdf Nikon Microscope Instruction Manual (Optiphot)]
  +
*[https://www.amscope.com/software/AmScope/MU-Series-Complete-Manual-Complete.pdf AM Scope manual link].
  +
*[https://www.amscope.com/software-download AM Scope Software link].

Revision as of 12:55, 3 December 2019

Microscopes
Microscopes.jpg
Tool Type Inspection, Test and Characterization
Location Bay 3, 6 & 7
Supervisor Tony Bosch
Supervisor Phone (805) 893-3486
Supervisor E-Mail bosch@ece.ucsb.edu
Description Optical Microscopes
Manufacturer Nikon & Olympus


Microscope Training - Use this guide for general usage info & the many advanced features available on most of our microscopes.

Nikon

The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.

Microscope #2: Nikon Optiphot 200 (Bay 7)

  • Trinocular: Ocular Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
    • DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #3: Nikon Eclipse L200 (Bay 6)

  • Manual for Nikon Eclipse L200D
  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #4: Nikon Eclipse L200D (Bay 6)

  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Olympus

The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.

Photo of Microscope #01 in the Characterization lab, Room 1111
Microscope #01 in the Characterization lab, Room 1111

Microscope #1: Olympus BHMJL (Char. Lab, Room 1111)

  • Trinocular: Ocular Binoc. + Camera (Exclusive)
  • LED Illuminator, Variable
  • Objectives: 10x, 20x, 50x, 100x
  • Bright/Dark Field
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for offline measurements: Link here.
  • User Manual: Olympus BHM Microscopes - Instruction Manual (PDF)

Microscope #5: Fluorescence Microscope: Olympus MX51 (Bay 6)

  • See the wiki page for the Olympus MX51 for full details
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Native Olympus Stream Software:
    • Photo/video capture
    • Calibrated measurement (calibrations locked)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top (Episcopic) & Bottom (Diascopic) Illumination
  • Three Fluorescence Filters (requires training, see main tool page for specs.)

Microscope #6: DUV Microscope: Olympus MX61A-DUV (Bay 7)

Please see the main tool page for detailed info on this microscope: Deep UV Optical Microscope (Olympus) 

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized Stage + Objective Turret
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  • Filters: to be added
  • Native Olympus MX61 Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
  • Deep-UV Light source + DUV Camera
    • DUV-100x sub-micron imaging/measurement

Olympus LEXT Confocal Microscope (Bay 4)

See the main tool page for complete info: Laser Scanning Confocal M-scope (Olympus LEXT) 

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized stage + Objective Turret
  • 100mm wafer stage
  • Native Olympus OLS2000 Software & Built-In Camera:
    • Calibrated measurement (calibrations locked)
    • Image stitching capabilities
  • 3D Laser-Scanning Confocal Microscopy capability:
    • 3D Topographical measurement (optical profilometry)
    • Surface roughness estimations (large roughness)
    • Thin-Film Film-Thickness Measurements (thicker films)

Procedures & Documentation

Retrieved from "https://wiki.nanofab.ucsb.edu/w/index.php?title=Microscopes&oldid=156979"