Difference between revisions of "Fluorescence Microscope (Olympus MX51)"

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(About)
(updated details / FLuorescence filters)
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=Detailed Specifications=
 
=Detailed Specifications=
  +
* Digital image capture & calibrated measurement via integrated Olympus Streams Essentials software.
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* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
* Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
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* Filters via rotatable selector:
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** '''BF''': Bright Field normal top-illuminated imaging
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** '''DF''': Dark-Field imaging
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** '''DIC''': Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
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* Fluorescence Filters (see [https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/fluorescence/anatomy/fluoromicroanatomy/ Olympus tutorial]):
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** '''''You must contact Tony Bosch for training on Fluorescence before using this feature!!!'''''
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** '''WB''': "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
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** '''WG''': "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
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** '''WU''': "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+
   
 
=Documentation=
 
=Documentation=

Revision as of 15:39, 18 December 2018

Fluorescence Microscope (Olympus MX51)
Fluorescence.jpg
Tool Type Inspection, Test and Characterization
Location Bay 6
Supervisor Tony Bosch
Supervisor Phone (805) 893-3486
Supervisor E-Mail bosch@ece.ucsb.edu
Description
Manufacturer Olympus


About

System for general purpose imaging. The system has bright field, dark-field, and DIC imaging modes. Screen captures and measurements on samples can be made through use of Olympus' Streams Essential Software, installed on the attached computer.

Detailed Specifications

  • Digital image capture & calibrated measurement via integrated Olympus Streams Essentials software.
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
  • Filters via rotatable selector:
    • BF: Bright Field normal top-illuminated imaging
    • DF: Dark-Field imaging
    • DIC: Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
  • Fluorescence Filters (see Olympus tutorial):
    • You must contact Tony Bosch for training on Fluorescence before using this feature!!!
    • WB: "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
    • WG: "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
    • WU: "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+

Documentation