Difference between revisions of "Microscopes"

From UCSB Nanofab Wiki
Jump to: navigation, search
m (Fluorescence Microscope: Olympus MX51 (Bay 6))
(Added Scope7: Olympus DSX1000)
 
(16 intermediate revisions by 4 users not shown)
Line 2: Line 2:
 
|picture=microscopes.jpg
 
|picture=microscopes.jpg
 
|type = Inspection, Test and Characterization
 
|type = Inspection, Test and Characterization
|super= Tony Bosch
+
|super= Demis D. John
 
|location=Bay 3, 6 & 7
 
|location=Bay 3, 6 & 7
 
|description = Optical Microscopes
 
|description = Optical Microscopes
|manufacturer = Nikon (3) & Olympus (1)
+
|manufacturer = Nikon & Olympus
}}
+
}}
  +
  +
== Procedures & Tools ==
  +
===[[UCSB NanoFab Microscope Training|'''Microscope Training Guide''']]===
  +
The guide linked above explains general usage info & the many advanced features available on most of our microscopes. New users are encouraged to read this, and also request in-person training by [[Demis D. John|Demis]].
  +
  +
=== Image Analysis Software ===
  +
Many of our microscopes have cameras and software for image capture. If you know the ''microscope and objective'' used for acquiring a photo, you can make calibrated measurements on the photos at your own desktop using the following software:
  +
*[https://www.amscope.com/software-download AmScope Software] - microscope image analysis software
  +
**[[Amscope Quickstart Usage Guide]]
  +
**AmScope Calibration File containing calibrations for all NanoFab microscopes: [https://wiki.nanotech.ucsb.edu/wiki/Images/uploads/2020/AmScopeCalsAll.magn Download Here]
  +
**Also available on '''''Nanofiles-SFTP / Manuals / Amscope'''''
  +
*[https://fiji.sc FIJI] - scientific image anaylsis software
  +
**[[FIJI - Microscope Measurement Tools|The Microscope Measurement Tools plugin]] has pre-configured calibrations for NanoFab microscopes & SEMs, and allows you to draw length measurements.
  +
***''Calibrations in this plugin repository are out of date as of microscope upgrades in 2019''.
  +
**There are many [https://imagej.net/Category:Plugins other useful plugins], for particle counting, [https://stackoverflow.com/questions/6230353/how-to-create-gif-animation-from-a-stack-of-jpgs/37193012#37193012 creating animations] etc.
  +
 
==Nikon==
 
==Nikon==
  +
The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.
The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7.
 
   
  +
===Microscope #2: Nikon Optiphot 200 (Bay 2)===
The scopes are connected to computers via Jenoptik ProgRes software which allows users to digitally capture images through the microscope and make calibrated measurements.
 
   
  +
*Trinocular: Ocular Binoc. + Camera (Simultaneous)
=== Microscope #2: (Bay 7) ===
 
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
* Trinocular: Ocular Binoc. + Camera (Simultaneous)
 
  +
*Filters: Green, ND
* ProgRes video/photo capture & measurement
 
  +
*Bright/Dark Field
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
  +
*Differential Interference Contrast (DIC/Nomarski)
* Filters: Green, ND
 
  +
**DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
* Bright/Dark Field
 
  +
*Top Reflected Illumination (Episcopic)
* Differential Interference Contrast (DIC/Nomarski)
 
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
** ''The DIC prism is installed on each objective and can't be removed, so even regular microscope images suffer from a slight double-image''
 
* Top Illumination
 
   
=== Microscope #3: Eclipse Nikon L200D (Bay 6) ===
+
===Microscope #3: Nikon Eclipse L200 (Bay 6)===
* [https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D]
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
 
* ProgRes video/photo capture & measurement
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Filters: Green, ND
 
* Bright/Dark Field
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Top Illumination
 
   
  +
*[https://wiki.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D]
=== Microscope #4: (Bay 6) ===
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
+
*Trinocular: Binoc. + Camera (Mutually Exclusive)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
* ProgRes video/photo capture & measurement
 
  +
*Filters: Green, ND
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
  +
*Bright/Dark Field
* Filters: Green, ND
 
  +
*Differential Interference Contrast (DIC/Nomarski)
* Bright/Dark Field
 
  +
*Top Reflected Illumination (Episcopic)
* Differential Interference Contrast (DIC/Nomarski)
 
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
* Top/Bottom Illumination
 
  +
  +
===Microscope #4: Nikon Eclipse L200D (Bay 6)===
  +
  +
*Trinocular: Binoc. + Camera (Mutually Exclusive)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
   
 
==Olympus==
 
==Olympus==
   
  +
The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.
=== [https://www.nanotech.ucsb.edu/wiki/images/2/28/Nikon-Microscope-OptiPhot-Manual.pdf Microscope #1: Olympus Optiphot-2 (Bay 3)] ===
 
  +
[[File:Microscope 01 Olympus BHMJL crop.png|alt=Photo of Microscope #01 in the Characterization lab, Room 1111|thumb|200x200px|Microscope #01]]
* ''To be added''
 
   
=== [[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]] ===
+
===Microscope #1: Olympus BHMJL (Bay 4)===
* See the wiki page for the [[Fluorescence Microscope (Olympus MX51)|Olympus MX51]]
 
* Trinocular: Binoc. + Camera (Simultaneous)
 
* Native photo/video capture & calibrated measurement
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Filters: Green, ND
 
* Bright/Dark Field
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Top/Bottom Illumination
 
* Three Fluorescence Filters
 
   
  +
*Trinocular: Ocular Binoc. + Camera (Exclusive)
=== [[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 7)]] ===
 
  +
*LED Illuminator, Variable
''Motorized Stage/Turret, Software control with Calibrated measurements, Deep-UV Light source+Camera for DUV-100x high res. imaging/measurement.''
 
  +
*Objectives: ''10x, 20x, 50x, 100x''
  +
*Bright/Dark Field
  +
*Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  +
*AMScope 14MP Cameras with calibrated software for measurements. Software available for offline measurements: [https://www.amscope.com/software-download Link here].
  +
*User Manual: [https://wiki.nanotech.ucsb.edu/wiki/index.php/File:Olympus_-_BHM_Microscope_-_Instruction_Manual.pdf Olympus BHM Microscopes - Instruction Manual (PDF)]
   
== [[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope]] ==
+
===Microscope #5: [[Fluorescence Microscope (Olympus MX51)|Fluorescence Microscope: Olympus MX51 (Bay 6)]]===
  +
  +
*See the wiki page for the [[Fluorescence Microscope (Olympus MX51)|Olympus MX51]] for full details
  +
*Trinocular: Binoc. + Camera (Simultaneous)
  +
*Native Olympus Stream Software:
  +
**Photo/video capture
  +
**Calibrated measurement (calibrations locked)
  +
*Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  +
*Filters: Green, ND
  +
*Bright/Dark Field
  +
*Differential Interference Contrast (DIC/Nomarski)
  +
*Top (Episcopic) & Bottom (Diascopic) Illumination
  +
*Three Fluorescence Filters (requires training, see main tool page for specs.)
  +
  +
===Microscope #6: [[Deep UV Optical Microscope (Olympus)|DUV Microscope: Olympus MX61A-DUV (Bay 4)]]===
  +
Please see the main tool page for detailed info on this microscope: [[Deep UV Optical Microscope (Olympus)]]
 
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
 
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
The LEXT has a motorized stage, image stitching capabilities and some image measurement capabilities, in addition to the primary topographical measurement capabilities afforded by the laser-scanning confocal microscopy function.
 
   
  +
*Motorized Stage + Objective Turret
See the main tool page for more info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]]
 
  +
*Trinocular: Binoc. + Camera (Simultaneous)
  +
*Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  +
*Filters: ''to be added''
  +
*Native Olympus MX61 Software control & Camera
  +
**Calibrated measurements (calibrations locked)
  +
**Z (focus) measurement via motorized stage height
  +
*Deep-UV Light source + DUV Camera
  +
**DUV-100x sub-micron imaging/measurement
  +
  +
=== Microscope #7: [[Digital Microscope (Olympus DSX1000)|Olympus DSX1000 Digital Microscope (Bay 4)]] ===
  +
Please see the main tool page for detailed info on this microscope, click the link above.
  +
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Supervisor to get training.
  +
  +
*Motorized Stage + Objective Turret
  +
*Digital Viewing via Computer
  +
*Objectives: High-Res.: 5x, 50x // Long-Distance + Angled Viewing: 10x, 40x
  +
*Filters:
  +
**Bright Field
  +
**Dark Field
  +
**DIC/Nomarski
  +
*Native Olympus Software control & Camera
  +
**Calibrated measurements (calibrations locked)
  +
**Z (focus) measurement via motorized stage height
  +
**Rapidly capture multiple imaging modes
  +
**Offline Analysis Software available for free
  +
*Tilted Imaging capabilities, for deep (> few micron) features.
  +
  +
==[[Laser Scanning Confocal M-scope (Olympus LEXT)|Olympus LEXT Confocal Microscope (Bay 4)]]==
  +
See the main tool page for complete info: [[Laser Scanning Confocal M-scope (Olympus LEXT)]]
  +
YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  +
  +
*Motorized stage + Objective Turret
  +
*100mm wafer stage
  +
*Native Olympus OLS2000 Software & Built-In Camera:
  +
**Calibrated measurement (calibrations locked)
  +
**Image stitching capabilities
  +
*3D Laser-Scanning Confocal Microscopy capability:
  +
**3D Topographical measurement (optical profilometry)
  +
**Surface roughness estimations (large roughness)
  +
**Thin-Film Film-Thickness Measurements (thicker films)
  +
  +
==Procedures & Documentation==
   
  +
*[[UCSB NanoFab Microscope Training|'''Microscope Training''']] - General procedures and info for using our microscopes
==Documentation==
 
* [[UCSB NanoFab Microscope Training|Microscope Training]] - General procedures and info for using our microscopes
 
   
*[[media:Nikon-Microscope-Manual.pdf|Nikon Microscope Instruction Manual (Ecplise)]]
+
*[//wiki.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Nikon Microscope Instruction Manual (Ecplise)]
*[[media:Nikon-Microscope-OptiPhot-Manual.pdf|Nikon Microscope Instruction Manual (Optiphot)]]
+
*[//wiki.nanotech.ucsb.edu/wiki/images/2/28/Nikon-Microscope-OptiPhot-Manual.pdf Nikon Microscope Instruction Manual (Optiphot)]
  +
*[https://www.amscope.com/software/AmScope/MU-Series-Complete-Manual-Complete.pdf AM Scope manual link].
*[[media:Jenoptik-ProgRes-Manual.pdf|Jenoptik ProgRes User Manual]]
 
  +
*[https://www.amscope.com/software-download AM Scope Software link].

Latest revision as of 11:35, 9 April 2021

Microscopes
Microscopes.jpg
Tool Type Inspection, Test and Characterization
Location Bay 3, 6 & 7
Supervisor Demis D. John
Supervisor Phone (805) 893-5934
Supervisor E-Mail demis@ucsb.edu
Description Optical Microscopes
Manufacturer Nikon & Olympus



Procedures & Tools

Microscope Training Guide

The guide linked above explains general usage info & the many advanced features available on most of our microscopes. New users are encouraged to read this, and also request in-person training by Demis.

Image Analysis Software

Many of our microscopes have cameras and software for image capture. If you know the microscope and objective used for acquiring a photo, you can make calibrated measurements on the photos at your own desktop using the following software:

Nikon

The Nikon scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.

Microscope #2: Nikon Optiphot 200 (Bay 2)

  • Trinocular: Ocular Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
    • DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #3: Nikon Eclipse L200 (Bay 6)

  • Manual for Nikon Eclipse L200D
  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #4: Nikon Eclipse L200D (Bay 6)

  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Olympus

The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.

Photo of Microscope #01 in the Characterization lab, Room 1111
Microscope #01

Microscope #1: Olympus BHMJL (Bay 4)

  • Trinocular: Ocular Binoc. + Camera (Exclusive)
  • LED Illuminator, Variable
  • Objectives: 10x, 20x, 50x, 100x
  • Bright/Dark Field
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for offline measurements: Link here.
  • User Manual: Olympus BHM Microscopes - Instruction Manual (PDF)

Microscope #5: Fluorescence Microscope: Olympus MX51 (Bay 6)

  • See the wiki page for the Olympus MX51 for full details
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Native Olympus Stream Software:
    • Photo/video capture
    • Calibrated measurement (calibrations locked)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top (Episcopic) & Bottom (Diascopic) Illumination
  • Three Fluorescence Filters (requires training, see main tool page for specs.)

Microscope #6: DUV Microscope: Olympus MX61A-DUV (Bay 4)

Please see the main tool page for detailed info on this microscope: Deep UV Optical Microscope (Olympus)

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized Stage + Objective Turret
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  • Filters: to be added
  • Native Olympus MX61 Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
  • Deep-UV Light source + DUV Camera
    • DUV-100x sub-micron imaging/measurement

Microscope #7: Olympus DSX1000 Digital Microscope (Bay 4)

Please see the main tool page for detailed info on this microscope, click the link above.

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Supervisor to get training.
  • Motorized Stage + Objective Turret
  • Digital Viewing via Computer
  • Objectives: High-Res.: 5x, 50x // Long-Distance + Angled Viewing: 10x, 40x
  • Filters:
    • Bright Field
    • Dark Field
    • DIC/Nomarski
  • Native Olympus Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
    • Rapidly capture multiple imaging modes
    • Offline Analysis Software available for free
  • Tilted Imaging capabilities, for deep (> few micron) features.

Olympus LEXT Confocal Microscope (Bay 4)

See the main tool page for complete info: Laser Scanning Confocal M-scope (Olympus LEXT)

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized stage + Objective Turret
  • 100mm wafer stage
  • Native Olympus OLS2000 Software & Built-In Camera:
    • Calibrated measurement (calibrations locked)
    • Image stitching capabilities
  • 3D Laser-Scanning Confocal Microscopy capability:
    • 3D Topographical measurement (optical profilometry)
    • Surface roughness estimations (large roughness)
    • Thin-Film Film-Thickness Measurements (thicker films)

Procedures & Documentation