Difference between revisions of "Microscopes"

From UCSB Nanofab Wiki
Jump to: navigation, search
m (update LEXT location, other minor updates)
(Microscope #2: Nikon Optiphot 200 (Bay 7): Deleted Jenoptic references, added AM Scope refs and links. Still needs table for pixel calibtrations versus mag for each scope....)
Line 10: Line 10:
 
The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7.
 
The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7.
   
The scopes have 3rd party cameras that are connected to computers via Jenoptik ProgRes software which allows users to digitally capture images through the microscope and make calibrated measurements.
+
The scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.
  +
  +
Calibrations versus mag are indicated in the table below for all scopes.
   
 
=== Microscope #2: Nikon Optiphot 200 (Bay 7) ===
 
=== Microscope #2: Nikon Optiphot 200 (Bay 7) ===
 
* Trinocular: Ocular Binoc. + Camera (Simultaneous)
 
* Trinocular: Ocular Binoc. + Camera (Simultaneous)
* ProgRes video/photo capture & user-editable calibrated measurement
 
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Filters: Green, ND
 
* Filters: Green, ND
 
* Bright/Dark Field
 
* Bright/Dark Field
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Differential Interference Contrast (DIC/Nomarski)
  +
** DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
** ''The DIC prism is installed on each objective and can't be removed, so even regular microscope images suffer from a slight double-image''
 
 
* Top Reflected Illumination (Episcopic)
 
* Top Reflected Illumination (Episcopic)
  +
* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
   
 
=== Microscope #3: Nikon Eclipse L200 (Bay 6) ===
 
=== Microscope #3: Nikon Eclipse L200 (Bay 6) ===
 
* [https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D]
 
* [https://www.nanotech.ucsb.edu/wiki/images/8/80/Nikon-Microscope-Manual.pdf Manual for Nikon Eclipse L200D]
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
* ProgRes video/photo capture & user-editable calibrated measurement
 
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Filters: Green, ND
 
* Filters: Green, ND
Line 31: Line 32:
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Top Reflected Illumination (Episcopic)
 
* Top Reflected Illumination (Episcopic)
  +
* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
   
 
=== Microscope #4: Nikon Eclipse L200D (Bay 6) ===
 
=== Microscope #4: Nikon Eclipse L200D (Bay 6) ===
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
 
* Trinocular: Binoc. + Camera (Mutually Exclusive)
* ProgRes video/photo capture & user-editable calibrated measurement
 
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 
* Filters: Green, ND
 
* Filters: Green, ND
Line 40: Line 41:
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Differential Interference Contrast (DIC/Nomarski)
 
* Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
 
* Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  +
* AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. [https://www.amscope.com/software-download Link here].
   
 
==Olympus==
 
==Olympus==
Line 92: Line 94:
 
*[[media:Nikon-Microscope-Manual.pdf|Nikon Microscope Instruction Manual (Ecplise)]]
 
*[[media:Nikon-Microscope-Manual.pdf|Nikon Microscope Instruction Manual (Ecplise)]]
 
*[[media:Nikon-Microscope-OptiPhot-Manual.pdf|Nikon Microscope Instruction Manual (Optiphot)]]
 
*[[media:Nikon-Microscope-OptiPhot-Manual.pdf|Nikon Microscope Instruction Manual (Optiphot)]]
  +
*[https://www.amscope.com/software/AmScope/MU-Series-Complete-Manual-Complete.pdf AM Scope manual link].
*[[media:Jenoptik-ProgRes-Manual.pdf|Jenoptik ProgRes User Manual]]
 

Revision as of 14:46, 23 July 2019

Microscopes
Microscopes.jpg
Tool Type Inspection, Test and Characterization
Location Bay 3, 6 & 7
Supervisor Tony Bosch
Supervisor Phone (805) 893-3486
Supervisor E-Mail bosch@ece.ucsb.edu
Description Optical Microscopes
Manufacturer Nikon & Olympus


Nikon

The lab currently has 3 Nikon Microscopes (Microscopes #2, #3 & #4). These are located in bays 6 and 7.

The scopes have 3rd party cameras that are connected to computers via AM Scope software which allows users to digitally capture images through the microscope and make calibrated measurements.

Calibrations versus mag are indicated in the table below for all scopes.

Microscope #2: Nikon Optiphot 200 (Bay 7)

  • Trinocular: Ocular Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
    • DIC only available on 100x and 150x mags due to image quality reduction at low mags due to DIC prisms which were removed for low mags.
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #3: Nikon Eclipse L200 (Bay 6)

  • Manual for Nikon Eclipse L200D
  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected Illumination (Episcopic)
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Microscope #4: Nikon Eclipse L200D (Bay 6)

  • Trinocular: Binoc. + Camera (Mutually Exclusive)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top Reflected (Episcopic) & Bottom Transmission (Diascopic) Illumination
  • AMScope 14MP Cameras with calibrated software for measurements. Software available for desktop measurements. Link here.

Olympus

The lab has two general purpose Olympus microscopes, and two specialized microscopes that require training to use, although both of these can also be used for general imaging.

Microscope #1: Olympus Optiphot-2 (Bay 3)

  • To be added

Fluorescence Microscope: Olympus MX51 (Bay 6)

  • See the wiki page for the Olympus MX51 for full details
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Native Olympus Stream Software:
    • Photo/video capture
    • Calibrated measurement (calibrations locked)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Filters: Green, ND
  • Bright/Dark Field
  • Differential Interference Contrast (DIC/Nomarski)
  • Top (Episcopic) & Bottom (Diascopic) Illumination
  • Three Fluorescence Filters (requires training, see main tool page for specs.)

DUV Microscope: Olympus MX61A-DUV (Bay 7)

Please see the main tool page for detailed info on this microscope: Deep UV Optical Microscope (Olympus)

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized Stage + Objective Turret
  • Trinocular: Binoc. + Camera (Simultaneous)
  • Objectives: 5x, 10x, 20, 50x, 100x, DUV-100x
  • Filters: to be added
  • Native Olympus MX61 Software control & Camera
    • Calibrated measurements (calibrations locked)
    • Z (focus) measurement via motorized stage height
  • Deep-UV Light source + DUV Camera
    • DUV-100x sub-micron imaging/measurement

Olympus LEXT Confocal Microscope (Bay 4)

See the main tool page for complete info: Laser Scanning Confocal M-scope (Olympus LEXT)

YOU ARE REQUIRED TO GET TRAINED on this tool before you are allowed to use it! Please contact the Tool Owner to get training.
  • Motorized stage + Objective Turret
  • 100mm wafer stage
  • Native Olympus OLS2000 Software & Built-In Camera:
    • Calibrated measurement (calibrations locked)
    • Image stitching capabilities
  • 3D Laser-Scanning Confocal Microscopy capability:
    • 3D Topographical measurement (optical profilometry)
    • Surface roughness estimations (large roughness)
    • Thin-Film Film-Thickness Measurements (thicker films)

Documentation