Difference between revisions of "Fluorescence Microscope (Olympus MX51)"

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|picture=Fluorescence.jpg
 
|picture=Fluorescence.jpg
 
|type = Inspection, Test and Characterization
 
|type = Inspection, Test and Characterization
|super= Tony Bosch
+
|super= Demis D. John
 
|location=Bay 6
 
|location=Bay 6
|description =  
+
|description = Optical Microscope
 
|manufacturer = Olympus
 
|manufacturer = Olympus
 +
|model = MX51
 
|materials =  
 
|materials =  
 
|toolid=
 
|toolid=
 
}}
 
}}
 
=About=
 
=About=
 +
System for general purpose imaging.  The system has bright field, dark-field, DIC imaging mode and Three fluorescence filters.  Screen captures and calibrated measurements can be made through use of native Olympus' Streams Essential Software, installed on the attached computer.
  
 
=Detailed Specifications=
 
=Detailed Specifications=
 +
* Native ''Olympus Streams Essentials'' software & Camera:
 +
** Digital image capture & calibrated measurement (calibrations locked)
 +
* Objectives: 5x, 10x, 20x, 50x, 100x, 150x
 +
* Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
 +
* Filters via rotatable selector:
 +
** '''BF''': Bright Field normal top-illuminated imaging
 +
** '''DF''': Dark-Field imaging
 +
** '''DIC''': Differential Interference Contrast "Nomarski" Imaging.  Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
 +
* Fluorescence Filters (see [https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/fluorescence/anatomy/fluoromicroanatomy/ Olympus tutorial]):
 +
** '''''You must contact Tony Bosch for training on Fluorescence before using this feature!!!'''''
 +
** '''WB''': "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
 +
** '''WG''': "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
 +
** '''WU''': "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+
  
 
=Documentation=
 
=Documentation=
 
*[[media:Olympus-Stream-Software.pdf|Stream Imaging Software Manual]]
 
*[[media:Olympus-Stream-Software.pdf|Stream Imaging Software Manual]]

Latest revision as of 11:09, 15 April 2020

Fluorescence Microscope (Olympus MX51)
Fluorescence.jpg
Tool Type Inspection, Test and Characterization
Location Bay 6
Supervisor Demis D. John
Supervisor Phone (805) 893-5934
Supervisor E-Mail demis@ucsb.edu
Description Optical Microscope
Manufacturer Olympus
Model MX51


About

System for general purpose imaging. The system has bright field, dark-field, DIC imaging mode and Three fluorescence filters. Screen captures and calibrated measurements can be made through use of native Olympus' Streams Essential Software, installed on the attached computer.

Detailed Specifications

  • Native Olympus Streams Essentials software & Camera:
    • Digital image capture & calibrated measurement (calibrations locked)
  • Objectives: 5x, 10x, 20x, 50x, 100x, 150x
  • Reflected (Episcopic) & Transmitted (Diascopic) Illumination modes
  • Filters via rotatable selector:
    • BF: Bright Field normal top-illuminated imaging
    • DF: Dark-Field imaging
    • DIC: Differential Interference Contrast "Nomarski" Imaging. Also requires "DIC-R" prism to be inserted. Polarization manually selected on DIC-R prism.
  • Fluorescence Filters (see Olympus tutorial):
    • You must contact Tony Bosch for training on Fluorescence before using this feature!!!
    • WB: "Blue" Fluorescence Filter. Excitation: 450-480nm / Dichroic 500nm / Barrier 515nm+
    • WG: "Green" Fluorescence Filter. Excitation: 510-550nm / Dichroic 570nm / Barrier 590nm+
    • WU: "Ultraviolet" Fluorescence Filter. Excitation: 330-385nm / Dichroic 400nm / Barrier 420nm+

Documentation