Difference between revisions of "Measurements and Imaging with Amscope Camera - Quickstart Usage Guide"

Revision as of 12:37, 26 August 2022

A number of our microscopes now have 14MP Amscope cameras with accompanying free Amscope software installed.

The software has already been calibrated for measurements on each microscope, users should not perform any calibrations without consulting Staff first.

Choosing the Calibration

In order for the software to know which objective you are using, you must choose the appropriately named Calibration from the dropdown lists. (eg. "5x, 10x, 20x etc.")

There are 2 separate ways to choose the calibration to use.

During live imaging

On the bar right above the image window, use the drop-down menu called Magnification, which is used for measuring live images. (This is also available from the top "Options" pull down menu). Choose the calibration from this list during live imaging, before clicking "Snap".

The Magnification dropdown menu.

Capture the Image:

Then, when the live image is ready, click [Snap] to capture an image. This image retains internal knowledge of which calibration was used during the capture.

After Image Capture

To change the calibration applied on an image that has already been captured, use the top menu bar item:

Menu "Image" > select "Resolution". Choose the correct calibration setting for the objective lens you were using and you will now have calibrated measurements for the still image.

Menu item Image > Resolution popup window with scope calibrations dropdown.

Saving an Image with Measurements

Perform measurements as desired using the various measurement tools (scale bars, line lengths etc.).

If you want the measurements saved onto the still image, use the menu item

Layers > Merge to Image... to merge the annotation layer onto the image. This will create a new image tab that has your measurements burned into it, the original editable tab is also still open.

Menu item Layers > Merge to Image... to burn the measurements into the image, before saving the image file.

The subsequent "Export options" window allows you to choose the Font size for the text etc.

Save your pictures as jpegs as normal.

You can save all open tabs into a folder with automatic naming using File > Batch Save.

@@ Line 3: / Line 3: @@
 The software has already been calibrated for measurements on each microscope, users should not perform ''any'' calibrations without consulting [[Demis D. John|Staff]] first.
-== Choosing the Calibration ==
+==Choosing the Calibration==
 In order for the software to know which objective you are using, you must choose the appropriately named Calibration from the dropdown lists. (eg. "5x, 10x, 20x etc.")
 There are 2 separate ways to choose the calibration to use.
-=== During live imaging ===
+===During live imaging===
 On the bar right above the image window, use the drop-down menu called '''Magnification''', which is used for measuring <u>live</u> images. (This is also available from the top "Options" pull down menu).  Choose the calibration from this list ''during'' live imaging, '''before''' clicking "Snap".
+[[File:AmScope - 02 annot.png|alt=screenshot of top menu bar|none|thumb|The Magnification dropdown menu.]]
+==== Capture the Image: ====
-Then, when the live image is ready, click [Snap] to capture an image.  This image retains internal knowledge of which calibration was used during the capture.
+Then, when the live image is ready, click '''[Snap]''' to capture an image.  This image retains internal knowledge of which calibration was used during the capture.
-=== After Image Capture ===
+===After Image Capture===
-To change the calibration applied on an image that has already been captured, use the top "'''Image'''" menu bar item and select "'''Resolution'''".  Choose the correct calibration setting for the lens you were using and you will now have calibrated measurements for the still image.
+To change the calibration applied on an image that has already been captured, use the top menu bar item:
+Menu "'''Image'''" > select "'''Resolution'''".  Choose the correct calibration setting for the objective lens you were using and you will now have calibrated measurements for the still image.
+[[File:AmScope - 06 chose mic2-150x annot.png|alt=screenshot of popup window|none|thumb|434x434px|Menu item '''Image > Resolution''' popup window with scope calibrations dropdown.]]
+==Saving an Image with Measurements==
 Perform measurements as desired using the various measurement tools (scale bars, line lengths etc.).
+If you want the measurements saved onto the still image, use the menu item
-== Saving an Image with Measurements ==
-If you want the measurements saved onto the still image, use the "'''Layers'''" pull down menu and '''Merge''' the layer to the image. This will create a new image tab that has your measurements burned into it, the original editable tab is also still open.
+'''''Layers''' > '''Merge to Image...''''' to merge the annotation layer onto the image. This will create a new image tab that has your measurements burned into it, the original editable tab is also still open.
+[[File:AmScope - Layers Merge - annot.png|alt=screenshot of menu item layers > Merge|none|thumb|Menu item '''''Layers > Merge to Image...''''' to burn the measurements into the image, before saving the image file.]]
+The subsequent "Export options" window allows you to choose the Font size for the text etc.
 Save your pictures as jpegs as normal.