Difference between revisions of "Measurements and Imaging with Amscope Camera - Quickstart Usage Guide"

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(copy/pasted BrianT's email from Jul 26, 2019)
 
(updated to indicate the 2 methods of choosing cals)
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A number of our microscope now have 14MP Amscope cameras with accompanying free Amscope software installed.
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A number of our microscopes now have 14MP Amscope cameras with accompanying [[Microscopes#Image Analysis Software|free Amscope software installed]].
   
The software has already been calibrated for measurements, users should not perform ''any'' calibrations without consulting Staff first.
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The software has already been calibrated for measurements on each microscope, users should not perform ''any'' calibrations without consulting [[Demis D. John|Staff]] first.
   
 
== Choosing the Calibration ==
 
== Choosing the Calibration ==
In order for the software to know which objective you are using, you must choose the appropriately named Calibration from the dropdown lists.
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In order for the software to know which objective you are using, you must choose the appropriately named Calibration from the dropdown lists. (eg. "5x, 10x, 20x etc.")
   
There are 2 separate spots where you choose the calibration to use. 
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There are 2 separate ways to choose the calibration to use. 
   
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=== During live imaging ===
One is on the bar right above the image window called '''Magnification''', which is used for measuring <u>live</u> images. (This is also available from the top "Options" pull down menu).
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On the bar right above the image window, use the drop-down menu called '''Magnification''', which is used for measuring <u>live</u> images. (This is also available from the top "Options" pull down menu). Choose the calibration from this list ''during'' live imaging, '''before''' clicking "Snap".
   
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Then, when the live image is ready, click [Snap] to capture an image. This image retains internal knowledge of which calibration was used during the capture.
After you have captured a still image, you use the top "'''Image'''" pull down menu and select "'''resolution'''".  Choose the correct calibration setting for the lens you were using (eg. "5x, 10x etc.") and you will now have calibrated measurements for the still image.
 
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=== After Image Capture ===
 
To change the calibration applied on an image that has already been captured, use the top "'''Image'''" menu bar item and select "'''Resolution'''".  Choose the correct calibration setting for the lens you were using and you will now have calibrated measurements for the still image.
   
 
Perform measurements as desired using the various measurement tools (scale bars, line lengths etc.).
 
Perform measurements as desired using the various measurement tools (scale bars, line lengths etc.).
   
== Saving an Image ==
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== Saving an Image with Measurements ==
If you want the measurements saved onto the still image, use the "'''Layers'''" pull down menu and '''Merge''' the layer to the image (this will create a new image that has the measurement data saved directly on it). 
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If you want the measurements saved onto the still image, use the "'''Layers'''" pull down menu and '''Merge''' the layer to the image. This will create a new image tab that has your measurements burned into it, the original editable tab is also still open.
   
 
Save your pictures as jpegs as normal.
 
Save your pictures as jpegs as normal.

Revision as of 16:18, 22 April 2020

A number of our microscopes now have 14MP Amscope cameras with accompanying free Amscope software installed.

The software has already been calibrated for measurements on each microscope, users should not perform any calibrations without consulting Staff first.

Choosing the Calibration

In order for the software to know which objective you are using, you must choose the appropriately named Calibration from the dropdown lists. (eg. "5x, 10x, 20x etc.")

There are 2 separate ways to choose the calibration to use. 

During live imaging

On the bar right above the image window, use the drop-down menu called Magnification, which is used for measuring live images. (This is also available from the top "Options" pull down menu). Choose the calibration from this list during live imaging, before clicking "Snap".

Then, when the live image is ready, click [Snap] to capture an image. This image retains internal knowledge of which calibration was used during the capture.

After Image Capture

To change the calibration applied on an image that has already been captured, use the top "Image" menu bar item and select "Resolution".  Choose the correct calibration setting for the lens you were using and you will now have calibrated measurements for the still image.

Perform measurements as desired using the various measurement tools (scale bars, line lengths etc.).

Saving an Image with Measurements

If you want the measurements saved onto the still image, use the "Layers" pull down menu and Merge the layer to the image. This will create a new image tab that has your measurements burned into it, the original editable tab is also still open.

Save your pictures as jpegs as normal.

You can save all open tabs into a folder with automatic naming using File > Batch Save.